How can precisely obtain a dillution with 1 bacteria per ml? There is standard inocullum with 1 bacteria /ml?

Answer 1

1.What bacteria are we talking about (E.coli? Which strain?)
2.There is no such standard solution for any type of bacteria.
3.Why on earth would you need such a dilution?
4.What type of facilites do you have access to (You can isolate single cells by FACs provided you have a proper marker and this expensive equipment)