suppose you perfrom a graim stain on a sample from a pure culture of bacteria and observed a field of purple?

Question

cocci. adjacent cells were not always the same color. what do you conclude?

Answer 1

That someone's biology homework is due in tomorrow?

Answer 2

Well firstly its Gram stain. If you have gram postive cocci they could be many things. Staphylococci, Steptococci, yeast, Candidan albicans there is loads of things it could be. If its has come out Garm postive (blue) then the cell wall must be mostly peptocgliycan. Dont be worried about the fact that the cells are not the same colour some of them take up the dye better. I would be tempted to do one of 2 things.

Catalase test: See weather the cell produces Catalase which can narrow down the speices

API test : this is probley better but its expensive, this test tells you the proable species

hope that helps

Answer 3

Gram-positive bacteria have a thicker peptidoglycan wall and stain purple while the more common gram negative bacteria contain less peptidoglycan and do not retain the purple coloured-dye.

This is becuase bacterial cell walls are negatively charged and bind to positively charged (basic) dyes such as crystal violet.
Bacteria which retain the primary stain crystal violet after further treatment with acetone are known as Gm + ve. Bacteria which loose the purple colouration and simply stain red are termed gram –ve

The bacterial cell wall is characterised by the presence of Peptidoglycan: A network of polysaccharide molecules connected by polypeptide links.In some bacteria, this forms a very distinct network around the outer surface of the cell (Gram-positive). In others, it is confined to a thin sandwich between two plasma membranes (Gram-negative)

Research peptidoglycan in bacteria cell wall... archaea don't have peptidoglycan at all and therefore this test can distinguish between eubacteria and archaea.

Hope this helps

Answer 4

I'd be wishing I'd listened to my lecturer and pretending I knew what the heck I was doing! I'd be checking what colour it should've been and got out matching nail polish bottle, adding a little to try and make it look right and then I'd blame it on the boy sitting next to me for sabotaging my experiment because I ran over his pet rat a week ago during my driving lesson.
Then I'd read the last part of the question and conclude that I should stick to Arts and Crafts after all.

Answer 5

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Answer 6

"RUN FOR IT"