I have a Chemicals Research application that involves use of high purity Triacetin. I intend to use Gas Chromatography technique to determine purity. Help on GC Column type and conditions would be appreciated. It is needed soon....It is top priority.
2006-11-15 02:10:21 · 2 answers · asked by Amit M 1 in Science & Mathematics ➔ Chemistry
Look up in Hand book of chemistry and mathematics, you school library will have it. Your professor should also have it.
2006-11-15 05:25:55 · answer #1 · answered by minootoo 7 · 0⤊ 0⤋
E. Jellum and P. Björnstad
Institute of Clinical Biochemistry, Rikshospitalet, University of Oslo, Oslo, Norway
Quantitative gas-liquid chromatographic determination of free glycerol in blood serum was accomplished through the use of butane-1,4-diol as internal standard. After removal of proteins with phosphotungstic acid, the glycerol and butanediol were acetylated for 1 hr with acetic anhydride. The acetates thus formed were extracted with diethyl ether, the solvent was removed, and the butanediol diacetate and glycerol triacetate were separated on a column with butanediol succinate as stationary phase at temperatures programmed from 150 to 190°. Normal sera were found to contain 0.4-1.2 mg of glycerol per 100 ml, the estimation having a range of ±5-10%. The method can be exploited to determine as little as 0.01 mg of glycerol per 100 ml when a sensitive flame ionization detector is used.
this method does not mention column type, but I would suggest you try a DB5
2006-11-15 12:30:32 · answer #2 · answered by Stonerscientist 2 · 0⤊ 0⤋