Why did crystal violet dyes inhibit growth of gram positive bacteria?

Question

why did the growth of gram positive bacteria is inhibited by crystal violet dyes in the selective media.

If "heat fixing" kills the gram positive bacteria it should also kills the gram negative bacteria.....then why only the growth of gram positive bacteria is inhibited while gram negative bacteria continue to grow in that culture (for eg in MacConkey Agar)

Answer 1

only dead bacteria can be stained. so when you put it on the slid and 'heat fix' it you kill the bacteria so therefore it can be stained. It's not the crystal violet that kills it.

Answer 2

simplest way is in all likelihood by way of using antibiotics that are bacteriacidal to gram +ve bateria and don't impact gram -ve micro organism. the ordinary distinction that this exploits is interior the form of their cellular partitions the difficulty is in simple terms in choosing an antibiotic which *purely* has an impact on gram +ve. the difficulty is that most of the extra uncomplicated solutions to this question (that persons could leap to) frequently have constrained action on gram -ve. There possibly would be suited antibiotics available to reply to this question. that's purely somewhat extra complicated than human beings could earlier each and every little thing presume through micro organism which characterize exceptions....even with the actuality that in step with risk at your point the strange exception would not count?

Answer 3

http://physchem.ox.ac.uk/MSDS/CR/crystal_violet.html

Answer 4

do u wanna know or u r tryin to use big diction............!