If there is a website with this information, I would greatly appreciate it! I'm trying to figure out the weight in nanograms of DNA per genome of my pathogen for an experiment. Thanks!!
2006-11-07 03:04:41 · 3 answers · asked by eternalstudent 1 in Science & Mathematics ➔ Biology
There is a simple approximation that the average molecular mass for a base pair is 660. That means that a mole of a base bair would be 660 g or if you prefer 6.023*10^23 base pairs would be 660*10^9 ng.
Therefore 1 ng would be (6.023*10^23)/(660*10^9) =9.13*10^11 base pairs. I am using base pairs since DNA is double stranded. (If you wanted to take into account the nucleotides of both strands then you just mutliply by 2 (18.26*10^11))
For calculating the mass of the DNA, if bp is the number of basepairs of the genome of interest then its approximate molecular weight is 660*bp and the mass is
mass=mole*MW = mole*660*bp
From here on it depends on how many moles of DNA you have. If you want to find out the weight of a single molecule it is MW/6.023*10^23= 660*bp/6.023*10^23 g= 660*bp/6.023*10^14 ng
2006-11-07 04:35:32 · answer #1 · answered by bellerophon 6 · 1⤊ 0⤋
Must know the molar mass of the substance in question in order to convert ng to nmol.
2016-05-22 07:28:00 · answer #2 · answered by Anonymous · 0⤊ 0⤋
Nanogramme
2016-12-14 14:23:46 · answer #3 · answered by ? 4 · 0⤊ 0⤋