2006-11-07 00:51:54 · 2 answers · asked by Saralili R 2 in Science & Mathematics ➔ Chemistry
Use the smallest mechanical pipette for the purpose, i.e., if you need to pipette 150 microliters, then use a 200 microliter pipette with tips to match. (I think these are correct scales; it has been awhile since I used the mechanical pipettes). I find a greater accuracy than trying to use a 1 ml pipette to aliquot 50 microliters of solution. I used a multiple mechanical pipette for ELISA plates; however, they often have one or more channel that does not fill properly on the first try. Therefore, I suggest, taking up, releasing and retaking the solution in the pipette until you visually verify the liquid levels in all tips are approximately equal.
On the whole, I have found that the retaking up of solution (take up aliquot, release, then refill the tip 1-3 times) makes for the most accurate takeup of the proper measured amount on the mechanical pipette.
2006-11-07 02:42:00 · answer #1 · answered by Black Dog 6 · 0⤊ 0⤋
A pipette repeater.
2006-11-07 01:08:33 · answer #2 · answered by j_rocketsauce 1 · 0⤊ 0⤋