from a culture tube to a cuvette, I need to transfer all of it, not just a sample
2006-11-05 08:31:34 · 3 answers · asked by si1enc3d 2 in Science & Mathematics ➔ Biology
I'm not rich so I can't get a laminar flow hood sorry
2006-11-05 08:45:42 · update #1
Do it in a laminar flow hood. Follow aseptic techniques.
Depending on your use, you may suspend your bacterial culture in an appropriate medium.
If you dont have a laminar flow hood, do it the traditional way...
wash your hands, then put alcohol.
work on a clean table - clean it with alcohol.
light an alcohol lamp in the area to clean air.
open your culture tube near the light.
transfer aseptically the bacterial culture to cuvette
- do it near light to ensure no contaminants from air
cover the cuvetter near the light.
2006-11-05 08:36:21 · answer #1 · answered by titanium007 4 · 1⤊ 1⤋
make sure you rinsed the bench top with alcohol or cleaning solution
pass mouth of culture over a flame
at 45 degrees to prevent airborne pollution, transfer your amount
dispense to cuvette
2006-11-05 09:22:41 · answer #2 · answered by Introverted White Waves 4 · 1⤊ 0⤋
Go back to school. Obviously you need more training.
2006-11-05 08:43:24 · answer #3 · answered by ? 2 · 0⤊ 3⤋