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Without using molecular methods in a bio lab, you would have to rely on phenotypic testing - ie. the expression of the gene.

in microbiology:
test the presence of the gene product and compare them to what is expected

Molecular methods would be more reliable and accurate in detecting a mutation. If you know the sequence of the normal gene you can amplify it by PCR and visualise it by agarose gel elecrophoresis - in which case, the mutated gene would give an altered product if any at all. Or you could use real-time-PCR with a labelled probe complementary to the gene sequence. During the PCR cycle, the probe will bind loosely to the mutated gene but strongly to the natural gene. So during the high temperature stages, the mix with the mutated gene will lose the signal from the probe before the mix with the natural gene.

There are other methods, but I'm not quite sure what you're dealing with...

2006-11-05 14:53:43 · answer #1 · answered by Nikoru 4 · 0 0

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