This is for animal cell culturing and i'm just touching the basics and i couldn't figure out the reason. Please help.
2006-10-28 18:57:28 · 3 answers · asked by a1ucardx 1 in Science & Mathematics ➔ Biology
When TC cells reach confluence they tend to go into a resting state since they cannot divide any more (nowhere to divide to). If you freeze cells when they are like this, they tend not to survive the freeze as well. Better to do it with the cells at 50% confluency. You'll end up using more flasks, but more of the cells will survive.
This is what I do. Some people I know freeze confluent cells and they swear it doesn't hurt their survival chances.
2006-10-28 19:27:49 · answer #1 · answered by Wally M 4 · 0⤊ 0⤋
breaks the cell tissue
2006-10-28 18:58:36 · answer #2 · answered by q6656303 6 · 0⤊ 0⤋
1.Growth becomes bad
2.dead some tissue
3.Sort a cheerful tissue
2006-10-28 19:08:57 · answer #3 · answered by nyansan 1 · 0⤊ 0⤋