What is the efficiency of a HPLC column?

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What is the efficiency of a HPLC column?

What is efficiency measured in? Low resolution suggests the column is efficient - how low is low?

2006-10-27 08:34:24 · 2 answers · asked by the forensics kid 1 in Science & Mathematics ➔ Chemistry

2 answers

The sharpness of chromatographic peak indicates the quality of the column. Actual equation for column efficiency is N = 16(tR/Wb)^2; N - is the number of theoretical plates, tR - retention time, and Wb - peak width at given peak height.

2006-10-27 09:02:02 · answer #1 · answered by titanium007 4 · 0⤊ 0⤋

Peaks really. See is there is any tailing. This indicates that your stationary phase is wearing out.

2006-10-27 10:45:43 · answer #2 · answered by cece 1 · 0⤊ 0⤋