2006-10-24 04:29:13 · 1 answers · asked by mmzr13 1 in Education & Reference ➔ Higher Education (University +)
From Web Site:
"Prepare sample(s) following one of the procedures below based on cell type.
Adherent Cells
Aseptically seed a sterile 25-cm2 flask (T-25) with approximately 5 x 104 cells in 10 ml of medium and grow for 2 population doublings (about 48 hours).
Without replacing the current culture medium, aseptically fill the flask completely with fresh culture medium. Seal the lid of the closed flask with Parafilm sealer.
Suspension Cells
Count cells.
Aseptically place approximately 2 x 105 cells in 10 ml of medium into a sterile container, either a small plastic tube with a screw-cap closure or a small flask. Seal the lid of the closed container with Parafilm sealer. "
2006-10-28 03:11:03 · answer #1 · answered by Teacher Man 6 · 0⤊ 0⤋