experiment of vitamin c?

Question

Can someone telll me how to determine the amount of vitamin c in a lemon and that in a vitamin c tablet?
I want the procedure and discussion(including error and improvement).
Thanks you so much!

Answer 1

You need a solution of DCPIP, and standard solutions of Vitamin C. You prepare a calibration curve and then you use this to estimate the vitamin C content of a lemon & a vitamin C tablet.

Get this from your library:

"Food and Health" Adds, Larkcom, Miller
Published by Nelson.

See page 86

But, this is a rather unreliable experiment, it is essential that you don't stir oxygen in to the mixture.

Answer 2

In a laboratory you might measure the amount of vitamin C in many different types of foods using a chemical reaction that is one of its functions in the body. Vitamin C (also called ascorbic acid) participates in chemical reactions in your cells oxidation-reduction reactions. Because of its function, vitamin C can react with iodine. You can measure the amount of vitamin C by adding iodine to food extracts until the vitamin C can bind no more iodine. Iodine in excess of the vitamin C will react with a starch solution you will add to the extract to produce a bluish-black color. The stepwise addition of a chemical (like iodine in this experiment) to measure another chemical such as vitamin C is called a titration. To make the measurement of the iodine added easier for you, you can use a measuring device called a burette. You will use the stopcock on the burette to slowly add iodine one milliliter (ml) at a time.
check out this website http://www.usd.edu/~bnoiva/vitc.htm

Answer 3

umm all i know is that the test for vitamin C is DCPIP, basically vitamin C is a powerful reducing agent and the DCPIP is a blue dye and adding the vitamin C decolourises the normally blue dye. umm i dunno how to find out how much vitamin C is in a lemon but the concentration can be determined by seeing how much of the how much of the vitamin C is needed to decolourise a standard solution of DCPiP, not sure if that helps but anyway good luck xXx

Answer 4

You can titrate a known amount of DCPIP dissolved in water with your sample dissolved in water to get the ascobic acid concentration. However DCPIP is a respiratory inhibitor so poisonous so if you are working in a school you might not be allowed to use it.

The other possibility is to titrate the ascorbic acid with an alkali your end point would be neutral pH.

Answer 5

Don't know about the lemon, but it should say on the bottle how much is in the tablet. Best also not to take too much per day, cos it just gets absorbed by the body. I take 1000mg per day, or 1500mg per day if I have a cold.

Answer 6

Sorry cant help you much but i do know that vitamin c does not stay in the body for any length of time, thats why we need so much of it.

Answer 7

it could be extraordinarily puzzling which you would be able to get rid of all materials of diet C out of your weight-alleviation plan, no longer to point "degree" the well being outcomes of a loss of diet C. there is extremely no way which you would be able to scientifically degree the outcomes on your physique from reducing out diet C. i will basically propose a Google seek.