what principle is protein precipitation based on?

Answer 1

Reducing the protein's solubility :)

The way it happens depends on the method of precipitation:

-Thermal. Enough energy is provided for the intramolecular interactions to be disrupted. The protein unfolds and hydrophobic patches are exposed. Such patches on different molecules interact with each other leading to aggregation and precipitation.

-pH. The net charge of the protein becomes zero at the isoelectric point. Then the molecules do not repel each other (they have no net charge) aggregate and precipitate. Also in some cases enough intramolecular ionic interactions and H-bonds are disrupted to cause denaturing.

-Adding compounds (salts or organic solvents). Based on the compound you can have denaturing of the protein and/or change in the water's properties as a solvent (effect of ionic strength, polarity, dielectric constant, chaotropic effects, etc).

Answer 2

The concept of Jesus as our redeemer has more to do with him serving as an ultimate version of an age old concept of redemption. In the Old Testament certain holy animals had to be sacrificed to the Lord. But God was not happy watching his creations die, especially since nobody was learning anything from that about what God wanted us to do. So Jesus was sent to the Earth to serve 2 main purposes: to teach God's will, and to serve as the ultimate sacrificial lamb for all mankind from Adam to the end of the world. Many atheists love to ask what happened to the souls of the jews who died before Christ came, the answer is that they were saved automatically because they didn't have the choice to accept the savior or not, this is also true of people today who die as young children.