Why a gene for antibiotic resistance is also inserted when human plasmid gene is inserted into the plasmid?

Question

when the human insulin gene is inserted into the plasmid, a gene for a antibiotic resistance is also inserted? why is this done?

Answer 1

generally a plasmid is a extra chromosomal DNA. when a gene (gene of interest)is isolated and iserted into the plasmid the plasmid goes is put into the E.coli and it is cultured. To identify the E.coli cells with the transformed gene, the plasmid along with the gene of interest a antibiotis resistant gene is also put. the process called as selection to identify the transformed E.coli ,all the E.coli cells are grown in the antibiotic only those cells that have the antibiotic resistant gene can grow ...so you select the cell and the gene in more (copies)

Answer 2

The gene for antibiotic resistance is not inserted at the same time; it pre-exists in the plasmid.

You grow th bacteria in the presence of the antibiotic in order to ensure that no other bacteria are growing apart from the ones that have the plasmid, but also to force the bacteria to keep the plasmid. In the absence of the antibiotic the cells will not bother to replicate the plasmid (since it won't be necessary for their survival) and after some generations it will be diluted out of the population.

Answer 3

the answer to #a million is my perfect buddy, DNA Ligase! hehe That one cements the backbone bonds between the insulin gene insert and plasmid. We use plasmids with antibiotic resistance because we use bacteria to clone (mass-produce) our altered plasmids. when we "rework" the bacteria, shoving plasmids into them, no longer all bacteria receives a plasmid. So, those non-plasmid bacteria will be killed off by technique of the antibiotic, leaving in difficulty-free words plasmid-bearing bacteria. i'm no longer particular what you're asking on question 7 precisely. Blunt end enzymes regularly are truly ineffective in cloning human being genes, when you consider that once you decrease open your plasmid to shove in the insert, your DNA ligase then has to connect both backbones of the DNA strands on both ends. because the ends are blunt with blunt-cutting enzymes, DNA ligase received't have the concepts of hydrogen bonds to get all of it mutually.

Answer 4

Human? For Bacteria, Selection.