What is meant by histochemistry and immune histochemistry?

Answer 1

Let's take it one step at a time. HISTOLOGY is the study of tissues, primarily by examining thin slices, or sections, of tissue with a microscope. However, such thin sections of tissue (typically about one cell thick) are virtually transparent, and to see them clearly you either have to use a special kind of microscope, such as a phase contrast microscope, or else increase the contrast of the tissue itself. This increased contrast is most often provided by reacting the tissue with various chemicals which will color different tissue components differently, thereby allowing them to be seen in a standard light microscope.

The science of preparing tissue slides for microscopic study is called HISTOCHEMISTRY. It is quite an involved field. Sometimes tissue is reacted with various dyes which will differentially stain different tissue components. As many as five or six dyes may be applied to the same tissue section, differentially staining five or six different components, such as chromatin, collagen, fibrin, mucin, elastin and cytoplasm. In other cases, rather than using a readymade dye, various reagents are used that will react with specific tissue components, producing a colored reaction products in situ.

One way of identifying tissue components is to use antibodies made against those components. For example, if you want to identify the location of myosin in a section of muscle you can make or purchase an anti-myosin antibody, and tag the antibody molecules with dye molecules. When you apply this tagged antibody to the tissue section, the antibody will attach very precisely to the myosin, and drag with it the dye. Then you can look in the microscope, and know that wherever you see the dye, myosin is present. Or the antibody can be tagged with a fluorescent compound and viewed with the fluorescence microscope. Or tagged with an enzyme, which is later reacted with a substrate to create a colored product. The use of antibodies to microscopically identify substances in tissue sections is called IMMUNOHISTOCHEMISTRY (all one word).

Answer 2

Immune histochemistry, is any subtance that helps in the decongestion of cold symptoms and flu that is naturally created by the immune system. Histochemistry is simply man-made synthesized substances that have the same effect.

That is to my knowledge anyway, using the term histo from anti-histamine

Answer 3

Histo=cell
Chemistry=what is going on in the cell from a chemical standpoint.

Immunohistochemistry studies the chemistry of the cell by using immuno markers such as goat and rabbit antibodies to tag for certain chemical changes that have taken place within a cell as a result of a treatment or drug administration. It is all done in vitro - not in vivo, so no worries.

Answer 4

Histology is another name for histochemistry. It is the study of tissue sectioned as a thin slice, using a microtome. It can be described as microscopic anatomy. Histology is an essential tool of biology.

Answer 5

Jackie Chan!