louis pasteur?

Question

what means of attenuation did pasteur use for his rabies vaccine?

Answer 1

This web-page should give you all the details :
http://en.wikipedia.org/wiki/Louis_Pasteur

Answer 2

Pasteur is particularly renowned for his work on the vaccine for rabies, a highly contagious infection which attacks the central nervous system. It enters the body through the bite of an infected animal or through infected saliva entering an existing wound. After experimenting with the saliva of animals suffering from the disease, Pasteur concluded that the disease rests in the central nervous system of the body. When an extract from the spinal column of an rabid dog was injected into healthy animals symptoms of rabies were produced. By studying the tissues of infected animals- rabbits, Pasteur was able to produce an attenuated form of the virus. This could be used for inoculation.

On July 6 1885, Pasteur tested his pioneering rabies vaccine on man for the first time. He saved the life of a young man called Joseph Meister who had been bitten by a rabid dog. Pasteur was urged to treat him with his new method. The treatment lasted 10 days and at the end he recovered and remained healthy. Since then thousands have been saved by this treatment.