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2006-10-16 09:12:19 · 6 answers · asked by pcg_100 2 in Science & Mathematics Chemistry

Ths questions relates to columns used in chromatography in general and not to specific chromatogrphic techniques

2006-10-16 09:33:40 · update #1

6 answers

temperature, flow rate, composition of the mobile phase (reaction of stationary phase with mobile phase can cause the column to degrade), pH

2006-10-16 17:49:07 · answer #1 · answered by laskoi 2 · 0 0

the langmuir isotherm can be used to predict the effects of chromatography conditions on separation. generally, the factors that determine column efficiency are: quality of resin; resin packing; size of column (all of the above relate to binding site availability). but factors such as temperature, pH, flow rate and ionic strength that are related to the chromatographic set-up rather than the column, will also affect performance.

2006-10-18 02:31:41 · answer #2 · answered by pinkebs00 2 · 0 0

it depends on the type of chromatography

for some pH gradient
size, ya what kind of column chromatography are you taking about

2006-10-16 09:16:56 · answer #3 · answered by dreson k 4 · 0 0

Solubility of your compound, the mobile phase used, the media, pH, length of column.

2006-10-17 01:28:07 · answer #4 · answered by sei 1 · 0 0

Also column length...size DOES matter!

2006-10-17 06:54:03 · answer #5 · answered by drjaycat 5 · 0 0

It depends on the solubility of the test subject in your solution

2006-10-16 09:42:38 · answer #6 · answered by SMG 3 · 0 0

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