function of RNase in the extraction of DNA?

Question

in the extraction of genomic dna,we used the rNase for what?

Answer 1

Use it to get rid of the RNA in the mix. Since you are analysing only DNA, you wouldn't want noise from the RNA floating around.

Answer 2

The addition of RNase is critical to obtaining pure DNA in your extraction; in general, purification of nucleic acids using standard techniques (such as DNA preparation kits) will yield both DNA and RNA, unless RNase is added to remove the RNA. Even though RNA is not as stable as DNA, there will still be some present if RNase is not added, and it will contaminate your prep. This is particularly problematic if you are interested in obtaining very pure DNA for sequencing--the RNA contamination may prevent accurate and effective sequencing (I know this from personal experience). In addition, when purifying DNA without RNase, you will see multiple RNA bands in your agarose gels (because RNA is also present), which can complicate further analysis. For example, if you are purifying a plasmid, you might not know which band is your plasmid. Or if you are purifying genomic DNA to look at DNA laddering in apoptosis, you might not be able to distinguish true DNA fragmentation from your RNA contaminants. Thus, it is best to just add the RNase. In commercial kits, I believe this is generally added in the lysis buffer. If you are also using proteinase K, you should probably add the RNase first; if you add the proteinase first, the RNase will likely be degraded before it can remove all of the RNA.

Answer 3

For alot of DNA techniques you dont need to go through the RNase step.......for example.alot of pcr techniques amplify specifically DNA not RNA.
RNase is to break down the RNA so that you only have DNA.

Answer 4

The extraction procedure will isolate total nucleic acids. Half the nucleic acid present will be RNA. To get rid of it you digest with RNAse which chops the RNA up into tiny pieces. These fragments can then be removed during ethanol precipitation.

Answer 5

Function Of Rnase

Answer 6

Please ask you genetic engineering teacher...if he couldnot answer, then please mail me...I will tell you in details