anyone know how to prepare a standard curve for acid oleic concentration?

Question

i have to prepare a oleic acid concentration standard curve in laboratory.................but i am not going to use enzymatic method as i do not have the equipment. anyone know how to do it?

Answer 1

First of all you need to define your experiment that you are going to use this standard curve for and the method you are going to use.

For example is oleic acid the final product or substrate of an enzymatic assay, is it just a component of a mixture and you are doing chemical analysis, what reagents are you using, is your quantification method spectroscopical, etc

In general you would dissolve known amounts of the compound in question (oleic acid in your case) in a proper solvent/solution corresponding to your assay. You take measurements of the standard solutions you prepared according to the method you want e.g. spectroscopy and make sure that for the range of concentrations you use you are within the linear scale of the method. You find the equation of the line using least squares method and calculate the R^2. The closer R^2 is to 1 the better the quality of your curve. If R^2<0.79 it is crap and you have to do it again.

You will get a good curve if you use at least 9 points, prepare the standards and do the whole procedure very carefully.

Answer 2

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Answer 3

extremely tough step. do a search in google or bing. just that can help!