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2006-10-09 23:17:39 · 3 answers · asked by hermesbg4 1 in Science & Mathematics Biology

3 answers

Elution is a term used mainly for purification of molecules (e.g. proteins, DNA, RNA) by column chromatography.
Let's assume you want to purify protein X from a cell lysate using a column (whatever type you want). You load your sample on the column and let it pass through the column. Your protein binds to the column while the others flow through. Now you have to retrieve protein X from the column. In order to do that, you have to pass a solution through the column that will disrupt the colun-protein X interactions, thus bringing protein X into solution.
For example for ion exchange you can use a solution of NaCl.
The retrieval of your desired molecule from the column with this procedure is called elution. So probably eluter refers to the solution you use to retrieve the molecule, though I've never heard of the term "eluter" before.

You also have elution for the retrieval of macromolecules from gels, which is usually combined with flow of an electric current (electro-elution). The apparatus for electro-elution could also be called eluter.

2006-10-10 07:18:40 · answer #1 · answered by bellerophon 6 · 0 0

never heard of that word before

2006-10-10 09:26:00 · answer #2 · answered by Anonymous · 0 0

is it related to boilogy?????????????????

2006-10-10 08:55:58 · answer #3 · answered by rockinsaint 2 · 0 0

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