2006-10-09 13:16:57 · 5 answers · asked by s a 1 in Science & Mathematics ➔ Chemistry
The standard deviation of the error increases enormously outside a particular range. This range will depend on the spectrophotometer or colorimeter you are using.
The calibration curve should be produced using values within the relatively reliable zone.
2006-10-10 00:25:08 · answer #1 · answered by Anonymous · 0⤊ 0⤋
Absorbance can go non-linear if the concentration is too much or too little. That means you would get the wrong answer if you used your calibration curve to determine a concentration from an absorbance that's not on it.
2006-10-09 20:52:46 · answer #2 · answered by rb42redsuns 6 · 0⤊ 0⤋
The relationship between concentration and absorbance is only linear over a certain range. The spectrometer will only accurately detect the absorbance over a certain range. Also the substrate that you are using must always be in excess otherwise you measure its concentration rather than the substance you are interested in.
2006-10-10 12:05:09 · answer #3 · answered by Ellie 4 · 0⤊ 0⤋
you can NOT extrapolate the curve
2006-10-09 20:19:26 · answer #4 · answered by Ralph 5 · 0⤊ 0⤋
do it urself
2006-10-09 21:23:38 · answer #5 · answered by aatif f 1 · 0⤊ 0⤋