2006-10-06 11:03:35 · 6 answers · asked by willo 1 in Science & Mathematics ➔ Biology
Blend a small amount of liver tissue and distilled water in a mini blender. Pour into a test tube and centrifuge it. The cellular components will separate into layers according to densities. I believe that the nuclei will be on the bottom and the cytoplasm will be on top. Look in a biology textbook to find what layer the mitochondria occupies. Siphon that layer and examine it under a microscope (high power).
2006-10-06 15:44:51 · answer #1 · answered by ursaitaliano70 7 · 1⤊ 0⤋
first, the liver cell membrane is dissolved by using a lipid solvent like Tween-20. then the protoplasn is centrifuged at 600 *g for 10 min when nucleus n membrane remnants settle down. the supernatant fluid is centrifuged at 7000-10000 *g for 10 min. the mitochondria now settles down which can b filtered n verified by addin its marker enzyme i.e,ATP Synthase(specific only to mitochondria).
2006-10-07 10:15:13 · answer #2 · answered by Anonymous · 0⤊ 0⤋
Perhaps you could break down the cytoplasm, and centrifuge the mixture, to seperate the mitochondria.
2006-10-06 19:18:55 · answer #3 · answered by Link 5 · 0⤊ 0⤋
bite the cell it will come out
2006-10-06 20:19:14 · answer #4 · answered by Rupesh 2 · 0⤊ 1⤋
by centrifugation
2006-10-07 07:44:24 · answer #5 · answered by dudu 1 · 0⤊ 0⤋
DNA
you're going to need a lab............
2006-10-06 18:12:27 · answer #6 · answered by miatalise12560 6 · 0⤊ 1⤋