2006-10-04 03:21:55 · 5 answers · asked by Aljeet 1 in Science & Mathematics ➔ Chemistry
This is all I can find on the subject
Separation of Plant Pigments Using Chromatography
http://www.ncsec.org/cadre2/team24_2/Plantpigments.htm
or maybe there is more information on this search
http://uk.search.yahoo.com/search?p=solvent+extraction+and+chromatography+plant+leaves+&prssweb=Search&ei=UTF-8&fr=FP-tab-web-t340&x=wrt&meta=vc%3D
hope it helps a bit :)
2006-10-04 03:35:47 · answer #1 · answered by Karen J 5 · 0⤊ 0⤋
we mash the leaves in a pestle and mortar with a few drops of ethanol to extract the chlorophyll then use paper chromatography with a solvent of 90:10 propan-1-ol:ethanol to separate the pigments. this works well on nettle leaves.
2006-10-04 08:08:54 · answer #2 · answered by Claire L 1 · 0⤊ 1⤋
Extract pigments from leaves by ether extration method.Seperate pigments by using acetic acid as a solvent and following the metod of column or paper chromatography. Use spectrophoto meter to measure the concentration of pigments. Go to library for more details.
2006-10-04 03:36:02 · answer #3 · answered by Pitambri 3 · 0⤊ 1⤋
I've done simple chromatography with my Bio students very easily. Seperating Chlorophyll a and B as well a rodophyll and xanthophyll.
2006-10-04 05:56:29 · answer #4 · answered by The Cheminator 5 · 0⤊ 1⤋
you're gonna first have to extract them from the leaves. using liquid N2 might help, but there are lit preps if you look for them
2006-10-04 05:10:59 · answer #5 · answered by shiara_blade 6 · 0⤊ 1⤋