If you used E coli DNA polymerase instead of Thermus aquaticus DNAA ploy. in PCR proced. what would be result?

Question

This would be in the PCR procedure. I need to know would the result be: PCR would be twice as fast, would PCR stop after 1 cycle, PCR would occur more slowly that normal, PCR not occur at all, or Many mistakes would be made??? Please help, I am trying to do research for a clinical and this is the only thing I am stuck on.

Answer 1

PCR would not occur at all, during the stage where the DNA dissociates, the E.coli DNA polymerase would denature and therefore would not be able catalyze the creation of DNA.