Can you think a way to prove: Amino Acid Sequence Determines Protein's 3D Shape/Structure?

Answer 1

The classic answer to this question was an experiment done with Ribonuclease, an enzyme that has its structure stabilized by a lot of disulfide bonds. The enzyme activity, which requires its shape to be correct, was determined. Then the enzyme was completely denatured by reducing the disulfide bonds and boiling it to separate all the non-covalent linkages. The enzyme at that point couldn't catalyze RNA breakdown at all. But, the investigators let it sit at room temperature for days, taking a sample to check for activity from time to time. Eventually it regained about 80 percent of its ability to catalyze the reaction. That made people think that the enzyme only needed its amino acid chain to figure out how to reconstitute its active shape. But this enzyme is not typical of all proteins, a lot need chaperones to help them fold, or require some kind of modification of amino acids to fold right, from clipping one or more peptide bonds as in insulin to adding chains of sugars for secreted proteins. It's a good idea that primary sequence determines three dimensional shape, it's just not always true.

Answer 2

different sequences of amino acids would result in disulphide bridges and hydrogen bonding in differnt places which would change the 3D/ tertiary shape/structure of the molecule.
Prove using enzymes mayb? they are specific and therefore have a specific 3D shape(and active site so will,usually, only bond with the substrate) sample of an enzyme and its substrate and another enzyme but using a the same substrate as before. ie amylase with starch, then a differnt enzyme with starch then perform the iodine test. from the amylase reaction u would see no colour change showing that a reaction has occured and broken down the starch, and from the other if no products should be formed as the enzyme has a different 3D shape due to the different sequence of amino acids and the iodine would turn from yellow/brown to blue/black. Indicating the presence of starch and showing that a different sequence of amino acids results in different 3D shapes. It mayb a load of rubbish ..but that's what i suggest do and am damn proud of my ingenius attempt lol

Answer 3

the dna, once transcribed to mRNA serves as the instructions for proteins, or at least sequences of amino acids. The ribosome assists in the production of proteins by providing a docking site for the mRNA. a type of transfer RNA(tRNA) has one end that is specific for an amino acid that it carries to the ribosome. on the other end, it has a site that is specific for a particular mRNA sequence(made up of 3 nucleotides mentioned earlier == codon). This tRNA's "anti-codon" does some base pairing with the sequence its specific for and then delivers the amino acid to the growing peptide chain. Now the ribosome in itself is a little more structured than this where the actually addition of the amino acids takes place, but i dont think you have to worry too much about it for now.

Answer 4

Of course, differing amino acids; different overall charge and many ways to be pulled into many differing shapes.