why is electron microscope specimens' thickness should be between 50-60nanometers?

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why is electron microscope specimens' thickness should be between 50-60nanometers?

2006-09-24 13:20:24 · 2 answers · asked by alaa m 1 in Science & Mathematics ➔ Physics

2 answers

You mean for transmission electron microscope. Too thin means not enough contrast (electron scatterings). Too thick, not enough electrons transmit (pass through) the specimen to be imaged.

2006-09-24 14:50:41 · answer #1 · answered by Dr. R 7 · 0⤊ 0⤋

Your hitting the specimen with electrons, and they won't penetrate too far into anything.

2006-09-24 20:29:19 · answer #2 · answered by Anonymous · 0⤊ 0⤋