what is a Pvu II?

Question

this is a Electrophoresis Lab. It has to do with DNA, buffer and Restriction Endonuclease.

Answer 1

Pvu II is a restriction enzyme from Proteus vulgaris that cuts DNA at CAGCTG

Answer 2

Pvu II is the name of a restriction enzyme, an enzyme which cuts specifically at the DNA sequence 5'-C A G^C T G-3' (this is double stranded DNA)

^ indicates the site of cleavage

Looks like this enzyme is what we call a "blunt-end cutting" enzyme because the resulting fragment would have a flat end with no bases sticking off. Many restriction enzymes leave a staggered "sticky" end.

To do a restriction enzyme "digest", you need to mix the DNA, water, buffer (usually diluted from a 10 x stock) and a few units of restriction enzyme. After an hour at 37C (98.6 F), the enzyme should have cut most if not all of the DNA molecules. The molecules are then separated by gel electrophoresis and the size of the fragments can be determined.

Answer 3

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