Molecular cell question? About stopping a protien from being transcribed?

Question

You would like to see the effect of shutting off the production of protien Z in the cell. You have at your disposal to alter the cell however you wish (in vitro experiment). How might you shut off the production of protien Z without altering transcription of the gene encoding for protien Z and without shutting off production of any other protein?

Answer 1

An antisense oligodeoxynucleotide (commonly called antisense oligo knock down) complimentary to the gene's mRNA transcript is one way of doing it but there are others. I need more information regarding the context of the question. Is this for an advanced molecular biology class or what?

Answer 2

Introduce a gene Y that when transcribed would pair up with the RNA encoding for protein Z, Y would hopefully have stop codons or some other defect to keep itself from binding with ribosomes.

Maybe also consider including genes to produce antibody to the ZY RNA complex.

(Its been years since I had studied this stuff, but its fun to express a thought on the subject.)

Answer 3

I think you can use Antisense RNAs which will have a sequence that is complimentary to the base sequence in mRNA. This will bind to the mRNA of protein Z. A double stranded RNA will be destroyed immediately. And, the protein production can be stopped.

Answer 4

Antisense RNA is one of the solutions.
Even better & more effective: transform the cells with small interferring RNA (siRNA) that is complimentary to Z RNA, but double stranded (hairpin shaped). In plant cells, the silencing is more effective if the loop of the siRNA hairpin is an intron.

Answer 5

i think it is related with the enzymes and stopcodon (UAG, UAA)