How can one prevent an enzyme,intolerant to low pH,from getting destroyed in a reaction where H+ are produced?

Question

suppose i am trying to study a reaction which an enzyme catalyzes. H+ form in the reaction but the enzyme gets destroyed at low pH. What could be added to the reaction mixture to protect the enzyme?

Answer 1

you should run the reaction in a buffer solution...
choose it according to the strength needed, the temperature, etc.

Answer 2

Oh dunce. Good choice on the name. Water has almost no affect on pH. Acid is low pH, not high (inverse log of the [H]). The reaction that produces H+ lowers pH making it more acidic not basic.

If the reaction can proceed in a buffered solution this is the best option.

Answer 3

What you need to do is to sop up the H+ ions as they are produced. You could add a buffer, which will react with them and prevent them from accumulating in the solution, so the pH will not go down to the forbidden zone. A typical buffer is Tris hydroxy amino methane, or you can use phosphate buffer or many others.

Answer 4

Add water to balance the pH level of the reaction. Water has a characteristic that can make it into an acidic or basic molecule. in this reaction, water would act as the acid which would increase the pH level, while the catalytic enzyme would produce the basic substance that loowers the pH level.

Answer 5

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Answer 6

In addition to the nice suggestion above, try to look for an appropriate buffer.