Distance traveled by compound of interest divided by distance traveled by solvent front (thus the value will be between 0 and 1).
2006-09-13 10:29:21
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answer #1
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answered by TheOnlyBeldin 7
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For a TLC plate, the mobile phase is a liquid, or at least a dissolved solid in solvent. A polar solvent will always yield a relatively higher Rf value than a non-polar solvent, and a non-polar substance will always yield a higher Rf value than a polar solvent. If the solvent is polar, then the substances are drawn up the plate by Van der Waals/London Dispersion forces or electrostatic dipole forces depending on the polarity of the molecule, and this is based on solubility and relative attraction between the plate and the solvent, from the point of view of the substance. Often times, the plate is silicone based, or in other words, very polar. The Rf value depends on the tendency of the substance to be attracted to, or likely in the stationary phase. Something more polar will be more attracted to the polar plate and will move up the plate less, a smaller Rf value. An analogous case would be in gas chromatography. The mobile phase is a gas, the stationary phase is a liquid. A less volatile liquid will have a higher tendency to stay in the stationary phase and will have a higher retention time, which works just like Rf value.
2016-04-10 05:09:10
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answer #2
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answered by Anonymous
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RE:
How do you find the Rf value on a TLC plate?
2015-08-10 16:54:45
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answer #3
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answered by Sybyl 1
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