Why do you need to let the mixture of bacteriophage T4 and e.coli to stand for 5 minutes?

Question

bacteriophage production,single step growth experiment

Answer 1

to permit bacteriophage inffects e. coli

Answer 2

The bacteriophage particles need to find their binding sites on the E. coli cells and stick on, then shoot their DNA into the bacterial cells. That takes five minutes approximately.

Answer 3

to allow phage penetration and integration into the host bacterium.