What would you expect when you had lysogenic virus?

Question

bacteriophage production,single step growth experiment

Answer 1

Lets correct your question first ---

1.there is no single “Lysogenic Virus”, rather there are many different type of viruses, thus you should type “Lysogenic Viruses”.

2.Your question CANNOT be answered with regard to “What should you expect …), if you don’t identify WHICH KIND of Lysogenic Virus”.

Having said that, let me give you some general info and a few good website.

In general any lysogenic virus --- “is a virus which have the capability to insert its DNA into the genome of the host bacterium for long-term dormancy, so that the bacterium replicates the viral DNA along with its own and passes it to its offspring. The virus is able to choose when it wants to reactivate and finish its lytic cycle, at which time it destroys the host and spreads its progeny to infect other bacterial cells.” [ Source: http://www.kcxo.com/Encyclopedia/thebiotechdictionary/lysogenic_virus.htm ]

Some of the viral genes may then be turned on and benefit the host cell. For example, in diphtheria, the gene that makes the toxin (poison) that is associated with the disease is from a lysogenic virus.

The lysogenized cell may keep the virus indefinitely and function with it or the inserted virus may detach itself and become lytic killing the host cell and releasing lots of virus particles.

Another example is the “human’s herpes virus associated with cold sores, known to have such a lysogenic relation with the skin of their lips. Years after a baby is infected, a child or adult may have a cold sore erupt.” [ Source: http://www.biotech-monitor.nl/3905.htm ]

According to http://www.biotech-monitor.nl/3905.htm :

{“
Lysogenic viruses: These viruses infect cells and integrate their genetic information into the genome of the hosts, leading to a permanent association as a ‘prophage’ with the cell and all its progeny. At intervals, it retrieves its genome from the host, replicates and finally breaks open the cell, setting new viruses free (lysis). The cycle of a lysogenic virus infection extends over several replications of the infected host cell. Occasionally, parts of bacterial DNA is carried along with the viral genome during the cutting-out process. This ‘transduction’ plays an important role in bacterial genetic exchange, spreading, for example, information for resistance properties. Therefore, and because of the long infection cycle, lysogenic phages are unsuitable candidates for phage therapy.
“}

The symptoms, or as you posed the question “What should you expect …”, depends on which kind of health problem you have (e.g., .HIV, Chickenpox, Human herpes, E. Coli, etc.)

For example, “Bacteriophage lambda is a lysogenic virus of E. coli, which can choose either the integrated or the lytic pathway.” [ Source: http://www.baylorhealth.edu/proceedings/12_3/12_3_dimijian.html ]

The symptoms of e. Coli is much different than HIV in your body. So, you streamline the problem, I am sure you can write a couple of line on symthoms.

Good Luck!

Answer 2

Apparently you're trying to decide how to do a single step bacterial virus growth experiment when the virus is lysogenic. That means it's attached to the chromosome of the bacterium in a silent state called lysogeny. To wake it up, you must shock the bacteria with heat, sonication, or some toxic chemical. The shock must inactivate a protein that's repressing the virus. Then, the lysogeny ends and you begin lysis. The virus pops out of the bacterial chromosome when it's awakened, steals the bacterial machinery to copy many more viruses (or it may make some of its own machinery), expresses its genes for the coat proteins and packages itself, then produces some proteins to lyse the bacterial cell and release the 100 to 400 virus or phage particles. The only part that's not like starting with virus is the initial shock, which substitutes for the virus/bacterial absorption step.

Answer 3

Andre Michel Lwoff
Andre Michel Lwoff (1902-1994), French microbiologist and winner of the 1965 Nobel Prize in physiology or medicine, which he shared with fellow countrymen Jacques Lucien Monod and François Jacob. The three men, all researchers at the Pasteur Institute in Paris, were recognized for their discoveries concerning genetic control of enzyme and virus synthesis.

Lwoff was born in Ainay-le-Chateau, France, on May 8, 1902. His parents were Russian émigrés. In 1921 he earned a B.S. degree from the University of Paris and began his medical studies, taking a part-time position as a research assistant at the Pasteur Institute. He received his medical degree from the University of Paris in 1927. Two years later, he was given his own laboratory at the Pasteur Institute. Lwoff also earned a Ph.D. degree from the University of Paris in 1932. He was named head of the Pasteur Institute's department of microbial physiology in 1938. In 1959 he became a professor of microbiology at the University of Paris. Lwoff continued his association with the Pasteur Institute until 1968, when he became director of the Cancer Research Institute at Villejuif, near Paris. He retired from the position in 1972.

After World War II (1939-1945) Lwoff, using bacteriophages (virus particles that infect and destroy bacteria) began his Nobel Prize-winning study of genes that control other genes. In a series of experiments, Lwoff showed that lysogenic bacteria (bacteria infected by bacteriophages) remained lysogenic from one generation to the next, proving that this cell-destroying trait is genetically determined and not the result of an external attack by bacteriophage. Lwoff found that the genes of the bacteriophage take over the genes of the infected bacteria, where they then orchestrate reproduction of the bacteriophage. He also detected a noninfectious form of bacteriophage, called the prophage, which inhibits reproduction of the bacteriophage within bacteria. He later discovered that the prophage could be activated through mutation caused by exposure to ultraviolet light, suggesting that cancer may be caused by mutating, and therefore activating, a dormant or latent virus. This was a controversial notion for many years, but one that eventually proved correct.

Lwoff is a member of the French Academy of Science and a foreign member of the United States National Academy of Sciences, the Royal Society of London, and the Soviet Academy of Medicine.

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Answer 4

1

Answer 5

a lot of mush....lysis is when the virus reproduces so much as to fill the cell and break the cytoplasmic membrane...so eventually the contents of all the cells would be spilled on to eachother and the process would begin....this is how the flesh eating virus works.

Answer 6

exploding bacteria and lots of virus! :o)

Answer 7

you expect your bacterial cells to "lyse", i.e., swell, explode and die.

Answer 8

uhh,,, i guess i'd expect my dr. to explain what it is