The major difference between the stains used in electron microscopy and those used in light microscopy is that

Answer 1

Whatever the stain used, it must change the kind of radiation you are using to "see" with.

In electron microscopy, the stain would be metal based, such as osmium tetroxide, because the heavy osmium atoms are "electron dense" - they deflect the electron beam & you see a dark area (transmission e.m.) or the sample surface (scanning e.m.)

In light microscopy, the stains are usually colored organic molecules, such as eosin or gentian violet, that show up very well in visible light to us color vision endowed humans.

Answer 2

Light microscopy stains typically stain based up molecular characteristics- eg there are specific stains for fats, proteins, carbohydrates, etc... Electron microscopy stains (for TEM) stain upon electron density. Gold and OsO4 are common

Answer 3

one shows up when viewed in electron microscopy and the other when viewed with a light microscopy

Answer 4

is that the answer is in your Biology book