2006-09-07 16:01:34 · 3 answers · asked by Rain 2 in Health ➔ Alternative Medicine
One- and two-stage radioligand assays were used to determine if human platelets possess the Lea antigen.^Goat IgG anti-Lea antibody was purified by multiple adsorptions with Le(a-b-) human red blood cells, followed by affinity chromatography with synthetic Lea substance and labeling with /sup 125/I.^Human IgG anti-Lea antibody was used either in a two stage radioassay with /sup 125/I-labeled mouse monoclonal IgG anti-human IgG as the second antibody or, alternatively, purified by Staph protein A chromatography, labeled with /sup 125/I, and used in a one-stage radioassay.^Platelets from donors of appropriate red blood cell phenotypes were incubated with the antisera, centrifuged through phthalate esters, and assayed in a gamma scintillation counter.^Dose response and saturation curve analysis demonstrate the presence of Lewis a antigen on platelets from Lea+ donors.^Furthermore, platelets from an Le(a-b-) donor incubated in Le (a+b-) plasma adsorb Lea antigen in a similar manner to red blood cells.^The clinical significance of these antigens in platelet transfusion remains undefined.
2006-09-07 16:07:54 · answer #1 · answered by Zsoka 4 · 0⤊ 1⤋
Lea Antibody
2017-01-14 03:24:21 · answer #2 · answered by ? 4 · 0⤊ 0⤋
how do get anti lea
2014-01-28 17:32:02 · answer #3 · answered by Stacy 1 · 0⤊ 0⤋