what is the structure of chikungunya virus?

Answer 1

Alright...search if from here yourself:

June 2006 - Origine and microevolution of the Chikungunya viruses responsible for the Indian Ocean outbreaks - Complete sequencing of the genomes of 6 viral strains


Researchers at the Institut Pasteur have managed to retrace the origin and evolution of the Chikungunya virus in the Indian Ocean through complete sequencing of the genome of six viral strains isolated from patients from Reunion Island and the Seychelles, as well as through partial sequencing of the viral protein E1 from 127 patients from the Indian Ocean islands (Reunion,Madagascar, Seychelles, Mauritius, Mayotte). Their study, published in PLoS Medicine, opens up new research paths that should help to explain the magnitude of the epidemic and the occurrence of severe forms of the disease.

For the first time ever, the complete genome sequences of the Chikungunya virus have been identified from viral isolates obtained from a low number of cell culture passages and therefore very close to the clinical virus, whereas to date the complete genomes identified were those of laboratory strains. This study, started at the National Reference Centre for Arboviruses, was continued on a large scale by the Institut Pasteur's technological platform Genotyping of Pathogens and Public Health . This work was undertaken in order to gain a better understanding of the origin and evolution of the viruses responsible for an epidemic of unprecedented magnitude. This outbreak started in early 2005 in Comoros, and has caused 248,000 cases in the Reunion Island between March 2005 and April 28, 2006 and 5834 cases in Mayotte, 8976 cases in the Seychelles, 6000 cases in Mauritius.

The study was supervised by Sylvain Brisse, head of the Pathogen Genotyping and Public Health Platform at Pasteur Genopole Ile-de-France, and Isabelle Schuffenecker of the Arbovirus National Reference Centre in Lyon, in collaboration with several teams from the Institut Pasteur in Paris, and with clinicians and virologists from the Reunion island, Madagascar (Institut Pasteur), Mayotte et the Seychelles.

The study shows that the Indian Ocean viral strains are closely related to each other and are related to East, Central and South African strains isolated between 1952 and 2000. The viruses that emerged in the Indian Ocean were therefore probably imported from the African continent. This scenario is compatible with the human population exchanges between East Africa and the Comoros where the epidemic started in early 2005. Moreover, changes that occurred in the viral genome as the epidemic progressed, especially the emergence and predominance of a particular genotype since September 2005, suggest an adaptive evolution of the viral strains.

Sequencing of the complete genome of a viral strain isolated from the cerebrospinal fluid (CSF) of a Reunion Island patient suffering from meningo-encephalitis revealed several mutations causing amino acid substitutions specific to this clinical isolate. Studies are currently underway to establish whether these substitutions are linked to the neurovirulence of the Chikungunya virus, on the one hand, and to greater efficiency of viral multiplication, on the other hand.

The viruses' " molecular signatures ", which constitute genetic fingerprints, in virus envelope protein E1 have also been found. As the three dimensional structure of E1 in a very similar alphavirus, the Semliki Forest virus, had already been ascertained by Felix Rey's team, this made it possible to provide a model of the structure of E1 of the Chikungunya virus in order to locate the mutations. One of these signatures(" E1 226 " : see figure below), not found early on in the epidemic, became predominant in Reunion Island strains from September 2005, and therefore shortly before the epidemic explosion. The authors suggest that this signature may confer an advantage and favour the multiplication of the virus in Aedes albopictus, its vector (protein E1 is in fact involved in attaching the virus to the mosquito's cell membranes).

The magnitude of the epidemic in the Indian Ocean and the description of new clinical froms of the disease has underlined "the critical lack of understanding of the physiology of the disease and the biology of the virus", as the authors point out.

The molecular data presented here is a first step to bridge this gap of knowledge and opens the way to functional studies. A dozen teams at the Institut Pasteur have indeed been working for several weeks on unveiling the mysteries of Chikungunya virus. Their research should, at middle-term, contribute to the development of tools to help combat this neglected disease.

Answer 2

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Answer 3

Chikungunya is caused by an alpha virus that is spread by mosquito bites.

Let me give you the website where the three dimensional model of this virus has been given.

http://www.pasteur.fr/actu/presse/press/06Chik_Ehtm.htm-10k

Answer 4

arent you talkin abt the "chicken guinea" virus...which is caused by the alpha virus or some such???