I want to prepare a standard solution of inorganic phosphate using KH2PO4 for estimation of inorganic phosphate in unknown sample by Fiske subbaroa method. I am not able to understand why 136 g of KH2PO4/liter should be taken and what is the concentration of Phosphate alone in this solution.
2006-09-01 06:11:51 · 2 answers · asked by Hara P 1 in Science & Mathematics ➔ Chemistry
136 g of KH2PO4 is 1 mole, and placed into a liter of liquid, the concentration of the resulting solution will be 1M in phosphate.
2006-09-01 07:28:58 · answer #1 · answered by Mr. E 5 · 0⤊ 0⤋
It has been possible to adapt the Fiske-SubbaRow method for phosphate so that alkaline phosphatase is determined by a second run, immediately following the determination of inorganic phosphate.
Studies of the effect of time, pH, and concentration of barbital on the enzyme activity are discussed
2006-09-01 14:43:04 · answer #2 · answered by ag_iitkgp 7 · 0⤊ 0⤋