Gram stain?

Question

In a Gram stain, one step could be omitted and still allow differentiation between gram-positive and gram-negative cells. What is that one step? Describe the appearance of the gram- positive and gram-negative cells if this step was omitted.

Answer 1

It's been a while, so I may not remember the reagents correctly.
The gram positive bacteria take the Gentian Violet stain. Once you fix that stain and decolorize the potentially gram negative bacteria you could identify the gram positive bacteria as those with color and the gram negative bacteria as those without color. The last stain in the process would stain those a red color. Hope that helps.

Answer 2

I am not sure that you could leave out a step. I do gram stains at work on a regular basis.

In a typical gram stain gram pos rods or cocci show up purple and negs show up pink. if you omit a step you will not get the staining that you need and cannot differentiate the two. ask your teacher but don't do it without his guidance. I would be curious to know what he thinks that step is. GL

Answer 3

.....? stain gram? gram stain? hellooo...? naybody home? nobody's home... she wants to go home but nobody's home...