At my job I do a lot of HPLC. While I understand most of the science behind it and behind the chromatograms, I have no idea what "plate count" means. Can someone enlighten me?
2006-08-30 14:07:38 · 6 answers · asked by jellybeanchick 7 in Science & Mathematics ➔ Chemistry
It has nothing to do with blood platelets, it has something to do with the peaks in the chromatogram.
2006-08-30 14:20:13 · update #1
Plate count is a (theoretical) measure of the efficiency of your column. You know that in chromatography there's an equilibrium between the stationary phase and the mobile phase; during elution there's a transfer of molecules from the mobile phase to the stationary phase and back to the mobile phase, and so on back and forth down the length of your column--that's how the separation occurs.
The distance along the column that it takes to make one of these transfers between phases and re-equilibrate is called the "theoretical plate height." If it takes less distance, that means the plates are narrow and you have more of them in the column, which means more transfers (and therefore equilibria) between phases can take place, and your column is more efficient. That of course means better resolution and better peak separation for you.
If it takes a comparatively greater distance for one phase transfer event, the plates are wider and there are fewer of them, so your efficiency (and therefore your resolution) is not as good.
The relationship between theoretical plate height, plate count, and column length is
theoretical plate height x plate count = column length
or
plate count = column length/ theoretical plate height
Remember, this is only theoretical, so round off any calculations you do for plate count. Hope this helps you.
2006-08-30 15:03:44 · answer #1 · answered by Schroedinger's cat 3 · 2⤊ 0⤋
Number Of Theoretical Plates
2016-11-08 06:12:13 · answer #2 · answered by ? 4 · 0⤊ 0⤋
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RE:
What does "plate count" mean in HPLC?
At my job I do a lot of HPLC. While I understand most of the science behind it and behind the chromatograms, I have no idea what "plate count" means. Can someone enlighten me?
2015-08-16 11:46:26 · answer #3 · answered by Anonymous · 0⤊ 0⤋
When they ask if it is a 51 reg plate they mean does it look like this: CA51 SIE If it does then it is a 51 reg, all it means is it was registered after/during September 2001. Like, a 57 reg car, CA57 SIE was registered this September, or after. It gives the potential buyer the chance to look up specifications on that model car, find out pros/cons and what the average mileage/price etc is.
2016-03-12 23:21:43 · answer #4 · answered by Sarah 4 · 0⤊ 0⤋
It's probably "theoretical plates". Any advanced physical chemistry text should have an exposition of them.
2006-08-30 14:48:13 · answer #5 · answered by rb42redsuns 6 · 0⤊ 0⤋
For the best answers, search on this site https://shorturl.im/axqcf
the car was first registerd between aug2001 and end of feb 2002
2016-04-05 02:48:20 · answer #6 · answered by Anonymous · 0⤊ 0⤋
Could it be referring blood platlets (sorry, don't know how to spell amd it's not in this spell checker!)
2006-08-30 14:14:45 · answer #7 · answered by Anonymous · 0⤊ 0⤋