I want stop / dormant the growth of bacillus culture (not to destroy) is am ammature lab assitant. Please help me in tis regard
2006-08-26 21:54:52 · 3 answers · asked by Sreekanth Reddy 1 in Science & Mathematics ➔ Biology
lowering the temperature is the best way to control the growth of most bacteria.
without more info on which bateria, it is difficult to give you more specifics, but if you want to use e coli at a relatively low concentration in an LB broth as an example-lowering the temp to 4C would slow the growth to a doubling rate of roughly 6-12 hours, vs a doubling time of maybe 20-30 minutes at 37C. lowering the temp of the same to -20C puts them nearly into stasis, lowering to -80C puts them completely in stasis. returning to higher temps from any of the lower temps will return the e coli to normal growth conditions, you will need to put something like glycerol into the solution if you are going to freeze and unfreeze them
2006-08-26 22:05:24 · answer #1 · answered by Anonymous · 1⤊ 0⤋
Lower the temperature.
2006-08-26 22:06:38 · answer #2 · answered by ag_iitkgp 7 · 0⤊ 0⤋
stick it in the fridge (@ 4 degrees celcius).
2006-08-27 05:30:23 · answer #3 · answered by sugar_lilly 3 · 0⤊ 0⤋