How can i purifiy all the heat shock proteins produced in a cell using affinity chromatography?

Question

What is heat shock proteins affinity to?

Answer 1

Not easy. A lot of the heat shock proteins help to refold heat-denatured unwound proteins, so one approach would be to use a column or beads with unwound proteins attached. But not all hs do this. Another approach would be to use bioinformatics to identify all the genes with a heat shock recognition element in their regulatory regoins (promoters, enhancers). Then you could look for common features that antibodies could recognize, but there might not be any. You could possibly isolate all such genes using affinity chromatography based on the heat shock regulatory element, and then transcribe and translate them in the test tube. But, I assume what you really want is to isolate them from a cell, maybe see how many of them are present. Very difficult to see how this could be done easily.