What is the different metween the male-specific phage and somatic phage?Can you detect them on the plate?How?

Question

It has to do with virus isolation and identification in raw sewage

Answer 1

Male specific phage are those bacteriophage that will only infect cells carrying an F-pillus and therefore harboring an F-episome. If you do a plaque assay on a petri dish, you will get plaques with F(+) bacteria and none with F(-) bacteria.


A male-specific phage infects bacteria via their pilius or pili (though asexual, bacteria with these appendages are often referred to as "male").

A somatic phage infects bacteria via their cell membrane.

Detection:
Since coliophages (those that infect E. coli) are most commonly male-specific, I assume this is the organism you will be using. The easiest way to detect for male-specific phages is look for the formation of plaques or lawns on susceptible strains of E. coli. Some E.coli hosts are also susceptible to somatic phages, so it is very import to select the correct bacterial host for this type of experiment.


ISOLATION OF RAW SEWAGE

http://highered.mcgraw-hill.com/sites/dl/free/0072487445/92720/Exercise37.pdf#search='virus%20isolation%20in%20raw%20sewage'

http://www.pubmedcentral.gov/articlerender.fcgi?artid=154486

Answer 2

yes i can dettect them , but why should i tell you. it's secret.