What is the oil immersion objective used in microbiological studies?

Question

Microbiology

Answer 1

Greetings;

The oil imersion is normally used for objective lenses >=100X. The reason for it's use is that it allows more light to enter into the objective lense, thus resulting in a sharper image. Essentially it's a drop of mineral oil placed on the slide that creates a thin layer of oil between the slide and the objective lense.

Answer 2

Low power objective lens can give magnification 10X. The eye piece can magnify it further at the most 15X . So the total magnification is at the most 150X.

High power objective gives magnification to the tune of 45X. So total magnification can go up to 675X.

Oil immersion lens , lens on the other hand has the power to magnify 100X . so the total magnification can be in the range of 3000X.

This is most convenient for the microbiology work as the bacteria are extremely minute and cannot be studied under smaller magnification.

The disadvantages of this lens are as follows;-

1) It requires maximum illumination of the field.

2) The preparation should be perfect as the depth of the field is almost Nil. In short thinner the slide /section the better.

3) The objective lens almost touches the cover slip and danger of damage to the slide or the lens is always there.

4) It requires a drop of ceder wood oil or equivalent between the lens and the objective.

So it requires an expert handling.

Answer 3

The oil immersion for microscope is ts simply for 100* more detail visualization....(way better image) :)

Answer 4

It varies. The oil objective used depends on what model of microscope you are using and what you are using it for.

Answer 5

The same question comes up again

Answer 6

Yeah it might be correct