2006-08-16 04:06:51 · 6 answers · asked by jatin 1 in Science & Mathematics ➔ Biology
HUH?
2006-08-16 04:11:19 · answer #1 · answered by ricky 2 · 0⤊ 1⤋
Tris Citrate Buffer
2016-12-13 04:02:46 · answer #2 · answered by hillyard 4 · 0⤊ 0⤋
temperature doesn't effect pH that much.
Because the ionisation is dependent on an energy function the temperature can effect it, but pH is a log function of the ionisation and so the change is very little.
Temperature would also increase the volume a small amount and this would decrease the concentration of H ions, increasing the pH slightly. But this amount is so small as to be irrelevent.
2006-08-16 04:27:37 · answer #3 · answered by heidavey 5 · 0⤊ 0⤋
It depends on the ambient temperature surrounding the buffer at the pH level withen 15 micro seconds, but most citrates would go through the inversion process at that point !!!!!!!? which would render them inoperative !!!!!!!!?
2006-08-16 04:25:07 · answer #4 · answered by john l 5 · 0⤊ 0⤋
temperature doesn't really affect the pH of citrate buffer. it's not like tris buffer where temperature can be an issue.
i've used citrate buffer for temperature optimum studies and it's fine.
2006-08-16 04:35:04 · answer #5 · answered by sugar_lilly 3 · 0⤊ 0⤋
Shofix supplies an extremely reliable link (the third one). "dpH(S)/dT usual replace of pH value according to degree centigrade. it extremely is constructive or unfavorable" finding on the reactions in touch (endothermic or exothermic) there will be a shrink or enhance of pH with a upward push of temperature. as an occasion for Tris-HCl you have dpH/dT=-0.028 pH gadgets/ degC, whilst for Tetraoxolate +0.070
2016-12-17 11:51:16 · answer #6 · answered by cheathem 4 · 0⤊ 0⤋