They are on an old plate (about a year or a little older) and contain a plasmid with kanamycin resistance. They don't seem to want to grow in LB or when spread on a fresh kan plate.
2006-08-15 04:57:36 · 5 answers · asked by cheetara_2001 2 in Science & Mathematics ➔ Biology
Try it in milk, or failing that ask an Agrobacteriaist.
2006-08-15 05:01:43 · answer #1 · answered by Anonymous · 0⤊ 1⤋
Does your LB contain kanamycin? Could be different resistance... Maybe try without antibiotic...
How are you spreading on a fresh plate? From one colony?
2006-08-15 05:03:03 · answer #2 · answered by mlevy79 2 · 0⤊ 0⤋
it grows very slow and is difficult to maintain. Add one of the following chemicals: Sucrose
Potassium Phosphate, Monobasic
Thiamine
Calcium Chloride, Dihydrate
1 M Magnesium Sulfate, Heptahydrate
Sodium Phosphate, Dibasic
Sodium Chloride
Ammonium Chloride
Agar
2006-08-15 05:03:47 · answer #3 · answered by babs1025 3 · 0⤊ 0⤋
Agrobacter radiobacter used to grow on Sheep blood agar held in either 10% Co2 or in a microaerophilc jar. It had to be left for approx 4 days to get any useful growth.
2006-08-16 01:26:34 · answer #4 · answered by slyno1300 1 · 0⤊ 0⤋
I grow mine in between my toes
2006-08-15 05:28:06 · answer #5 · answered by razzledazzle 2 · 0⤊ 0⤋