Anyone who is familiar with library construction is welcomed to give me some advices or opinion. I'm struggling with the library construction =s
2006-08-08 01:02:05 · 2 answers · asked by dna_supreme 2 in Science & Mathematics ➔ Biology
Ouch... You're in deep waters aren't you?
I've only heard about metagenomics so I don't really know the experimental complications with that. Haven't you done a literature search?
I haven't much experience with libraries. I guess it depends a lot on what type of library you're constructing. I have a friend who did a lot with phage display libraries so I am sure he can be more helpful. However I don't know whether he has time and I guess you should be more detailed about the experiment and your questions.
I can give you his e-mail if you want. Just contact me.
2006-08-08 01:42:24 · answer #1 · answered by bellerophon 6 · 1⤊ 0⤋
That's a big question! If it helps any, I've actually constructed several metagenomic libraries both from soil and wastewater treatment tanks (crazy environments, I know). How you go about library construction depends alot on your sample, what size insert you want, any downstream applications (sequencing, expression, hybridization, etc), and what vector you want to use.
If you have some specific questions or would like to bounce some ideas around, feel free send me an email (parsllc@auburn.edu) and we can talk. It's a pretty big subject!! :)
2006-08-08 20:51:21 · answer #2 · answered by Anonymous · 1⤊ 0⤋