What are the differences between Scanning and Electron Microscopy?

Question

Preferably in a tabulated manner, pertaining to use in Microbiology

I know the basic difference in principle.I need a much more detailed table of differences between SEM and TEM for Undergraduate Mirobiology level.

The qstn shouldh ave been DIFFERENCES BETWEEN SCANNING AND TRANSMISSION ELECTRON MICROSCOPY.Forgive the error.

Answer 1

Do you mean _optical_ and electron microscopy? Or do you mean _scanning electron_ microscopy and _transmission electron_ microscopy?

Optical microscopes use visible light to resolve the image, while electron microscopes use beams of electrons. Electron microscopes can achieve greater resolution (and therefore can resolve images of smaller objects) than optical microscopes can.

Scanning electron microscopes resolve an image by bouncing a beam of electrons off of the target (basically, front lighting). Transmission electron microscopes "shine" a beam of electrons through the target into a detector on the other side (back lighting) to create an electron silhouette.

Answer 2

Do you mean the differcences between transmission electron microscope and Scanning Electoron Microscopy?
or
Do you mean the differences between Confocal Scanning Microscopy and Electron Microscopy?

Please address your question clearly.

Answer 3

Scanning EM many times does not have adequate determination to make certain guy or woman proteins. TEM can get lots greater effective determination and the 1mm thick glass does not be a issue, in actuality thats the way you many times mount photographs... How super your protein is might desire to be an argument, i've got faith it should be a minimum of 40 KD to be seen. while you're in simple terms attempting to attempt for the presence of protein there are lots of much less complicated much less costly techniques, what precisely are you attempting to do?

Answer 4

The scanning is one of electron microscopes names, it scans the specimen in 2 dimensions, the other new one is tunneling scanning electron microscope it gives a 3 dimensional picture of the specimen.

Ahmad Barrasali
Biology lecturer