how to prepare 10mM HEPES buffer from 1M HEPES?

Answer 1

scared

Answer 2

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RE:
how to prepare 10mM HEPES buffer from 1M HEPES?

Answer 3

1 M = 1000 mM, so to prepare 10 mM Hepes, you need to dilute the stock solution 1/100.

For example, if you wanted to make 1 liter of 10 mM HEPES, you would use this recipe:

10 ml of 1M HEPES
990 ml of H20

The formula C1V1 = C2V2 is used in these instances
C1 = concentration of stock solution
V1= volume of stock solution
C2= concentration of final solution
V2 = volume of final solution

IN this case to make 1 liter of 10 mM HEPES in this formula:
(1000 mM) V1 = (10mM)(1000 ml)
Solving for V1 gives you 10 ml.

Keep practicing and you will get it.

Answer 4

So you want .010M HEPES. You jus dilute the 1M HEPES. So you actually have 1mol/Liter HEPES. You need to dilute it 1:100.
This is an option - Take 1mL of original solution and add 99 mLs of water. Use whatever unit you would like, but that was just an example.

Answer 5

You need to dilute

C1V1=C2V2=>

V1=(C2/C1)V2= 0.01/1*V2= 0.01*V2

So if you want to prepare V2 ml of 10mM just calculate the amount of ml (V1) of the 1M solution you need with the above formula.

DO NOT follow the advice of the other answers to add V2-V1 ml of water. When you are preparing decent amounts of volume, that is more than 2ml, always put the amount of concentrated solution in a volumetric flask/cylinder. add water up to V2 and mix.

ONLY when you are working in the range below 2ml and especially with microliters you do the approximation Vsolvent=V2-V1 for the dilution!!!!

Answer 6

Just dilute it based on the total volume.
For example:
If you want to prepare 10ml of 10mM HEPES.

You know your final concentration is 0.01M HEPES.
So for preparing 10ml of 0.01M HEPES, you need 0.1ml of 1M HEPES with 9.9ml of solvent (Water?)

Is it clear?

Answer 7

a milli mole is a thousandth of a mole. !0 millimoles is a hundredth.
If you want a litre of the new stock, then you would extract 10 millilitres of the 1M stock, add to a flask and add water until it measured exactly 1 litre. Dont take out 10mls and add 900 mls - different densities will affect final volume.

Answer 8

1m Hepes

Answer 9

yes no harm