2006-08-02 03:40:07 · 3 answers · asked by sanju1436 1 in Science & Mathematics ➔ Biology
Like genericman said it depends on the machine.
Most models tend to show up to 2.999.
However in order for you to get meaningful results you should dilute your samples so that OD never exceeds 1.0.
Above 1.0 you don't have linearity any more.
2006-08-02 04:52:53 · answer #1 · answered by bellerophon 6 · 0⤊ 0⤋
as genericman said it depends on the model.
max OD of most instruments is above 1.0. keep in mind that absorbance is the inverse log with respect to trasnmittance. Therefore abs of 1 = 10% transmittance. Abs of 2 = 1%. Precision (the repeatability of a reading) of most instruments falls off sharply over abs of 2. Therefore for most instruments absorbance values that are 1 or higher are not reliable anyway. That is why the absolute max OD of the instrument is not as important as the precision at high OD values.
2006-08-02 23:09:57 · answer #2 · answered by rezman 1 · 0⤊ 0⤋
You're at least the 2nd person to ask the same question.
What specific model of colorimeter are you using? Different models will have different limits.
2006-08-02 10:47:30 · answer #3 · answered by genericman1998 5 · 0⤊ 0⤋