ficoll hypaque used for density centrifugation to separate lymphocytes.n also density of red blood cells?
2006-07-28 05:23:04 · 1 answers · asked by Anonymous in Science & Mathematics ➔ Biology
well, its tough to figure out what you need.
Ficoll-Sodium Metrizoate is the chemical used... but also some of these use Percoll (an aqueous colloidal solution of polyvinylpyrrolidone-coated silica). So just search these 2 chemicals on yahoo for their densities.
If you are lookin for how to calibrate the test: get some Percoll, equilibrated to 300 mOsm with sodium chloride, was found to be the ideal gradient medium for density measurements of tissues with a density greater than 1.0 g/ml. which includes most types of lymph and parathyroid types... not sure about Lympho and Erythrocytes tho. Sorry!
2006-07-28 05:35:33 · answer #1 · answered by Peter Griffin 6 · 0⤊ 0⤋