What is a Dorner endospore stain, and what is the microscopic appearance after this procedure?

Question

In the Dormer endospore stain, a smear covered with carbolfuchsin is steamed, then decolorized with acid-alcohol and counterstained with nigrosin. What would the microscopic appearance be after this procedure?

Answer 1

The endospores inside each bacterium (or free-floating, of course, if they've already been lysed) will be a bright pink, while the bacteria themselves are a light pink. Here's a great illustration:

http://biosci.sierra.cc.ca.us/materials/4/illustrations/dorner_method.html

Hope that helps! ( :

Answer 2

Dorner Endospore Stain